Assignment 1
NMR Guided Reading
January 26, 2000
The first thing necessary to do is learn to control the spectrometer very
well. Having a few basic skills for optimally locking the instrument and
shimming the field to an extremely good homogeneity will help us immensely later
on. For this first exercise, we are going to hone your present skills in
these areas considerably.
Locking: Please read in Derome the section on "The deuterium
lock" on pages 44-46 and pay particular attention to the following
parameters/concepts: lock power, lock gain, lock phase, lock field, and
saturation of the lock signal. These are also discussed in Braun,
Kalinowski, Berger in Section 1.3 pp. 6-7.
For an optimum lock signal:
 | Lock power: We want this as high as possible without saturating our
signal. |
 | Lock gain: We want this as low as possible without risking losing
our lock signal. |
 | Lock phase: We want this as close to directly on phase as possible
to maximize the response from the lock circuitry (this provides a steadier
lock signal) |
 | Lock field: We need to adjust this correctly prior to trying to
obtain the lock (otherwise the automatic lock routine will be fouled up) |
 | Saturation is bad, bad, bad. This occurs when we are pumping to much
energy (via the lock power parameter) into the sample. This results in
the equalization of the spin states for the deuterium solvent and the lock
signal disappears. If the lock signal disappears, the instrument
cannot lock. |
After you understand those concepts in general, turn to page 120 of Derome
and read section 5.3.4. This gives a better picture of what the lock is
trying to do.
Now we are ready to look at the effects of varying these parameters.
Place in the spectrometer our standard H2O/D2O sample with
the white cap. Spin the sample and lock the sample up as you would
normally do.
Note the important parameters:
| |
Beginning |
Saturated |
Not Saturated |
Optimized |
| Lock power: |
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| Lock gain: |
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| Lock phase: |
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| Lock field: |
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Let's start with lock power.
- Increase the lock power until the signal starts to saturate. This is
indicated by instability in the lock signal (it starts waving up and
down). When this occurs, note the value of the lock power again.
- Play with the lock gain to see if this makes any difference in the
behavior, note your observations. Reset the gain to its original
value.
- Reduce the level of the lock power until it no longer is
saturating. Note the value.
- Now, watching very carefully drop the level of the lock power by 3 or 4
units and watch the lock signal. It should appear to drop, and then
slowly rise back up. Repeat this until dropping the lock signal by 2
or so units gives a steady signal (no upward climb). The reason that
we see this unsteady behavior is that we are still near the saturation
limit. When you can change the signal and get a completely steady
signal you are out of the saturation danger zone. Note the value.
- Our lock power is now optimized and we should now play with the lock
phase. The lock phase is dependent upon the lock power (i.e. changing
the lock power also changes the lock phase). This is because on old
spectrometers like ours, the amplifiers for power output are not linear in
their phase outputs. What that means for us, is that anytime we change
the lock power, we need to readjust the lock phase. Do that now by
simply adjusting the lock phase to achieve a maximum signal. Note the
new value.
- It is possible that if the lock phase changes enough that we may start
saturating our signal again. So, if there is a big change in lock
phase you need to check the lock power again for saturation. If it
changes then you have to adjust the lock phase again and so on (usually one
iteration is enough).
- After you have adjusted the lock phase, turn off the lock for a little bit
and look at the pseudo-fid of the lock. Notice how both waves are more
or less symmetrical and have the same phase. Relock the instrument.
- After these two parameters are set, we need to adjust the lock gain.
The lock gain amplifies the deuterium lock signal once it leaves the
probehead. The value of the lock gain does not affect signal
saturation or lock phase. The only thing that matters here is
decreasing the amount of noise in the signal while still maintaining a
strong enough lock signal to maintain the lock. Turn down the lock
gain until the lock is lost (light starts blinking) and then turn it up 3 or
4. Note the value. You should notice that the lock signal is now
a lot less noisy. This is better for the lock circuitry to
"see" small changes in field and correct for them. If there
is a lot of noise, bits of noise get counted as field changes resulting in
small constant shifts in the lock frequency and hence the spectrometer
frequency. This leads to broad ugly lines.
Shimming:
All right, the next part of the exercise is shimming. Shimming is the
art of homogenizing the magnetic field such that all nuclei in the NMR tube
experience exactly the same magnetic field (excepting of course, differences in
chemical shift, coupling, and gyromagnetic ratio). Read the sections in
Derome on shimming, Sec. 3.3.4 on pages 42-49. Don't worry about the bit
on shimming on the fid yet.
We will work on the spinning shims z, z2, and z3
first. Our z4 shim doesn't seem to act quite right so don't
touch it for now.
The shims and a reasonable shimming procedure are discussed in Braun,
Kalinowski, Berger (BKB) in Section 1.4, pp. 7-12.
- A good first step to the shimming process is to save what you've got right
now so that you can always get back to it if you really mess things
up. You should do that now. In the MacNMR software, pick out the
little NMR console button and save the shims under some new filename.
If things get fouled up during the shimming exercise below, just read in the
old values.
- You can now start shimming. This can be done either from the MacNMR
console or from the old Bruker console. I find the latter easier, but
maybe it is just cause I know it better. It really doesn't matter,
they are doing the same thing. If you are going to use the Bruker then
you need to turn off the console page and get back to the main program of
MacNMR (otherwise the Mac doesn't release control of the Bruker to
you). Either way you do it, you need probably to set the lock gain to
a higher value so that small changes in homogeneity result in large changes
in the signal (you can set it back down to our optimized level later).
- We are going to assume that the shimming is not so bad and therefore skip
the First Round the BKB describe. We will go straight on to the second
round steps 1 and 2. Follow the procedure to maximize the lock signal
as described. When the signal is maximized, note the values of the z,
z2, and z3 shims.
- Take a one-scan proton spectrum and expand around the signal. Does
it look pretty good? If not shim some more, otherwise print out the
expanded spectrum.
- When your shimming is good and you have a printed spectrum, deliberately
change z pretty drastically. Take a new spectrum and plot it
out. Your nice singlet for water should now be a doublet. Reset
z and repeat this procedure for z2 and z3 plotting as
you go. If you are interested, try changing z2 and z3
the other direction. How does that affect the spectrum differently?
- Reset all of your shims to their "good" values and turn off the
spinner. The level of the lock will probably drop, sometimes it drops
enough to lose your lock. This is because a substantial amount of
inhomogeneity in the xy plane is removed by averaging it by spinning the
sample. We can actually get reasonable homogeneity without spinning
the sample if we adjust the non-spinning shims. We should use steps
1-4 of the third round of shimming to fix the non-spinning shims. Do
so and then take another spectrum to compare. Note that z, z2,
and z3 will need to be fixed slightly if you are not spinning
(the sample moves up and down slightly when the normal cushion of air from
the spinner is absent).
- Turn spinning back on and then readjust z, z2, and z3 to optimize their
values. Take a spectrum. Now deliberately mess up the
"x" shim by A LOT. Take another spectrum. Notice how
there are now absolutely huge spinning sidebands. Measure the distance
between those spinning sidebands in Hz and verify that the difference is a
multiple of the spinning frequency.
That is the end of the first assignment. The next assignment will
involve learning all of the various dashboard parameters for MacNMR to allow
complete control over the acquisition of spectra.