|
Date
|
Text Readings
|
Paper for Discussion
|
| 8/28 |
introduction |
|
| 8/31 |
1-32: introduction |
Crick, F., and J. Watson. 1956. Structure of Small Viruses.
Nature 177:473-475.
|
| 9/2 |
33-50: phage attachment and
penetration |
|
| 9/4 |
50-57: attachment of animal
viruses |
1) WuDunn, D. and P.G. Spear. 1989. Initial Intereaction of
Herpes Simplex Virus with Cess is Binding to Heparan Sulfate.
J. Virol. 63:52-58.
2) Bergelson, J.M., et al. 1992. Identification of the Integrin
VLA-2 as a Receptor for Echovirus1. Science 255:1718-1720.
|
| 9/7 |
57-60: general mechanism of
penetration of animal viruses |
1)Fan, D.P., and B.M. Sefton. 1978. The Entry into Host Cells
of Sindbis Virus, Vesicular Stomatitis Virus, and Sendai Virus.
Cell 15:985-982.
2) Madshus, I.H., S. Olsnes, and K. Sandvig. 1984. Mechanism
of Entry into the Cytosol of Poliovirus Type 1: Requirement for
Low pH. J. Cell Bio. 98:1194-1200.
|
| 9/9 |
60-67: attachment and penetration
by animal viruses: HIV |
Maddon, P.J., et al. 1986. The T4 Gene Encodes the AIDS Virus
Receptor and is Expressed in the Immune System and the Brain.
Cell 47:333-347
|
|
| 9/11 |
no class meeting: groups meet
to prepare papers for presentation |
1) Feng, Y., et al. 1996. HIV-1 Entry Cofactor: Functional
cDNA Cloning of a Seven-Transmembrane G Protein-Coupled
Receptor. Science 272:872-877.
2) Deng, H., et al. 1996. Identification of a Major Co-Receptor
for Primary Isolates of HIV-1. Nature 381:661-666.
3) Dragic, T., et al. 1996. HIV-1 Entry into CD4+ Cells is
Mediated by the Chemokine Receptor CC-CKR-5. Nature
381:667-673.
4) Alkhatib, G., et al. 1996. CC CKR5: A RAMTES, MIP-1aMIP-1bReceptor as a Fusin Cofactor for Macrophage-Tropic
HIV-1. Science 272:1955-1958.
5) Lapham, C.K., et al. 1996. Evidence for Cell-Surface
Association Between Fusin and the CD4-gpl20 Complex in
Human Cell Lines. Science 274:602-605.
|
| 9/14 |
HIV attachment and penetration,
continued |
|
| 9/16 |
77-87: replicaton of T-series
coliphage; T-odd phi |
|
| 9/18 |
87-101: replication of T4, gamma
and phiX174 |
|
| 9/21 |
101-103: replication of RNA
phage |
1) Suglyama, T., and D. Nakada. 1970. Translational Control of
Bacteriophage MS2 RNA Cistrons by MS2 Coat Protein: Affinity
and Specificity of the Interaction of MS2 Coat Protien with the
MS2 RNA. J. Mol. Biol. 48:349-355.
2) Kastelein, R.A., et al. 1982. Lysis Gene Expression of RNA
phage MS2 Depends on a Frameshift During Translation of the
Overlapping Coat Protein Gene. Nature 295:25-41.
|
| 9/23 |
116-118: eukaryotic genetics
review |
|
| 9/25 |
118-125: positive-strand RNA
viruses: poliovirus |
1) Molin, A., A.V. Paul, and E. Wimmer. 1991. Cell-Free De
Novo Synthesis of Poliovirus. Science 254:1647-1651.
2) Lemm, J.A., et al. 1994. Polypeptide Requirements
for Assembly of Functional Sindbis Virus Replication
Complexes: a Model for the Temporal Regulation of
Minus- and Plus-strand RNA Synthesis. EMBO J..
13:2925-2924.
|
| 9/28 |
125-133: positive-strand RNA
viruses: togaviruses and coronaviruses |
Jacobs, L., et al. 1981. Synthesis of Subgenomic mRNA's of
Mouse Hepatitis Virus is Initiated Independently: Evidence from
UV Transcription Mapping. J. Virol.> 39:401-406.
|
| 9/30 |
133-135: positive-strand RNA
viruses: plant viruses |
Presentations: leader primed vs. discontinuous transcription
models of coronavirus replication
|
| 10/2 |
135-140: negative strand RNA
viruses: rhabdovirus |
Presentations: cleavage vs. re-initiation transcription
models of rhabdovirus mRNA synthesis
|
| 10/5 |
140-143: negative strand RNA
viruses: paramyxoviruses and orthomyxoviruses |
Galarza, J.M., et al. 1996. Influenza A Virus RNA-Dependent
RNA Polymerase: Analysis of RNA Synthesis in Vitro. J. Virol.
70:2360-2368.
|
| 10/7 |
143-155: orthomyxoviruses |
|
| 10/9 |
ambi-sense and double-stranded
RNA viruses |
|
| 10/12 |
155-158: retroviruses: reverse
transcription |
|
| 10/14 |
155-163: retroviruses: integration
and gene expression |
due: review of paper on RNA
virus replication |
| 10/16 |
EXAM |
|